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. 2021 Mar 8;49(11):e64. doi: 10.1093/nar/gkab144

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — RSF1 and NIPBL are recruited to sites of UV damage. (A) RSF1 and NIPBL are recruited at sites of UV damage in wild type cells upon 30 J/m² irradiation. Fibroblasts were locally irradiated using 5 μm pore filters and left for 4 h in no serum containing medium with HU (10 mM) and with (upper panel) or without (lower panel) EdU. After fixation cells were labelled with Alexa-azide and anti-RSF1 (upper panel) or with anti-γH2A.X and anti-NIPBL (lower panel). (B, C) RSF1 (B) and NIPBL (C) is recruited at the sites of DNA damage in wild type cells (upper panel) but not in XPA deficient cells (lower panel) upon 100 J/m² UV irradiation. Fibroblasts were locally irradiated using 5 μm pore filters and left for 1 hour in no serum containing medium with HU (10 mM). After fixation cells were labelled with anti-CPDs and anti-RSF1 (B) or with anti-XPG and anti-NIPBL (C). Scale bar: 12.5 μm.