Skip to main content
. Author manuscript; available in PMC: 2021 Jun 21.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Mar 5;32(4):996–1007. doi: 10.1021/jasms.1c00005

Figure 3.

Figure 3.

(A) Unaligned and (B) SVA summed mobility spectra of a mixture of four heavy-labeled peptide epimers. (C) SVA spectra of individual peptide epimers: (orange) LVFFAEDVGS[dD]K, (purple) LVFFAEDVGS[dbD]K, (light blue) LVFFAEDVGSDK, (dark red) LVFFAEDVGS[bD]K. Each spectrum is the sum of 25 separations acquired using a multipass SLIM. Compression ratio ion mobility programming (CRIMP)45 was performed after the 1st pass using a compression ratio of 50 000 to 1. TWsep speed = 320 m/s. TWsep amplitude = 40 V. Guard = 15 V. Injection time = 500 ms in-SLIM accumulation. Pressure ≈ 4.0 Torr helium.