Expanded views of negative Agilent tuning mixture ions acquired using 4 ion levels of a multilevel SLIM. (A) m/z 1034, (B) m/z 1334, (C) m/z 1634, (D) m/z 1934, (E) m/z 2234, and (F) m/z 2534. Each panel shows spectra of unaligned (red), SVA (green), and DTW (blue) processed data. (G) Full width at half max measurements for negative Agilent tuning mixture ions in unaligned (red), SVA (green), and DTW aligned (blue) mobility spectra. Pressure fluctuations were induced by opening and closing the door to the ion source housing. Each mobility spectrum represents a sum of 50 separations. TWsep speed = 192 m/s. TWsep amplitude = 27 V. TWesc speed = 24 m/s. TWesc amplitude = 24 V. Guard = −35 V. Injection time = 40 ms in-SLIM accumulation. Pressure ≈ 3.6 Torr helium.