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. Author manuscript; available in PMC: 2021 Jul 9.
Published in final edited form as: J Med Chem. 2020 Jun 9;63(13):7243–7251. doi: 10.1021/acs.jmedchem.0c00475

Figure 3.

Figure 3.

(A) Relative survivin protein level in different human prostate cancer cell lines determined using Western blot analyses. (B) IC50 of 7I in different human prostate cancer cell lines derived from dose-dependent survival curves (Figure S2) as determined using methylene blue assay. (C) Association of 7I IC50 with survivin expression level in different human prostate cancer cell lines. (D, E) Expression of HA-tagged survivin determined using Western blot analysis (D) and IC50 of 7I derived from survival curves (Figure S3) as determined using methylene blue assay (E) in stable C4-2 clone with overexpression of HA-tagged survivin (Sur) and vector-transfected (Vec) control cells. (***p < 0.001). (F) Effect of 7I on the expression of survivin, XIAP, CIAP1, and CIAP2 in C4-2 and PC-3 cells as determined using Western blot analysis.