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. Author manuscript; available in PMC: 2021 Jul 9.
Published in final edited form as: J Med Chem. 2020 Jun 9;63(13):7243–7251. doi: 10.1021/acs.jmedchem.0c00475

Figure 4.

Figure 4.

Compound 7I induces survivin degradation in the proteasome. (A) Time course of 7I-induced survivin loss in C4-2 and PC-3 cell lines determined using Western blot analysis. (B, C) Effect of 7I on survivin half-life. C4-2 and PC-3 cells were pretreated with cycloheximide (CHX) followed by chasing for different times in the presence of 7I or DMSO control. Cells were lysed for Western blot analysis of remaining survivin (B) and quantification of gel densities from three independent experiments (C). (D) Effect of proteasome inhibitors MG132 and bortezomib on 7I-induced survivin degradation as determined using Western blot analysis. Actin was used as a loading control. Quantification was from three independent experiments (**p < 0.01; ***p < 0.001).