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. 2021 Jun 4;17(6):e1009624. doi: 10.1371/journal.ppat.1009624

Fig 5. DH840.1 binds to HIV-1 Env in a mode distinct from C3/V4 neutralizing antibodies.

Fig 5

(A) 3D reconstruction of negative stain electron microscopy of DH840.1 Fab in complex with HIV-1 stabilized soluble CON-S envelope trimer shown from the top or side of the trimer (Left and Middle). Fitting of the HIV-1 Env trimer and DH840.1 Fab into the negative-stain EM map (Right). (B) Overlay of the negative-stain EM maps of stabilized soluble HIV-1 CON-S envelope trimer in complex with DH840.1 (colored orange), HIV-1 BG505.SOSIP Env trimer with BG505 autologous neutralizing antibody CP506 (blue; EMDB-9003), and HIV-1 Env with CD4 binding site bnAb VRC01 (gray; EMDB-6193). (C) Antibody binding titers as log AUC in ELISA to CON-S gp120 with (black) and without (orange) the N339 glycosylation site.