Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse- αTgTRPPL-2 | This work | Silvia Moreno (University of Georgia) | Recognizes TgGT1_310560 in T. gondii tachyzoites IFA (1:100) WB (1:1000) |
| Antibody | Rabbit-αSag1 | Mineo et al., 1993 | IFA (1:1000) WB (1:1000) |
|
| Antibody | Rabbit-αSERCA | Nagamune et al., 2007 | IFA (1:1,000) WB (1:1000) |
|
| Antibody | Rabbit-αGap45 | Gaskins et al., 2004 | IFA (1:1000) WB (1:1000) |
|
| Antibody | Mouse-αTubulin | Sigma Aldrich | Cat#11867423001 | WB (1:10,000) |
| Antibody | Mouse-αHA | Roche | Cat#11867423001 | IFA (1:1000) |
| Antibody | Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A20181 | IFA (1:1000) |
| Antibody | Alexa Fluor 546 | Thermo Fisher Scientific | Cat#A20183 | IFA (1:1000) |
| Cell line | ΔTgTRPPL-2 | This work | Silvia Moreno (University of Georgia) | Clonal cell line with the TgGT1_310560 disrupted Validated through genomic amplification, qPCR, IFAs, and WB |
| Cell line | TgTRPPL-2-smHA | This work | Silvia Moreno (University of Georgia) | Clonal cell line with the TgGT1_310560 gene locus tagged Validated through genomic amplification, IFAs, and WB |
| Cell line | ΔTgTRPPL-2-trppl2 | This work | Silvia Moreno (University of Georgia) | Clonal cell line with the TgGT1_310560 disrupted and expressing an extra copy of the same gene Validated through genomic amplification, qPCR IFAs, and WB |
| Cell line | RHTatiΔku80 | Sheiner et al., 2011 | Parental line used for tagging | |
| Recombinant DNA reagent | TgTRPPL-2-smHA | This work | Plasmid used to C-terminally tag TgGT1_310560 | |
| Recombinant DNA reagent | pMOS003-lenti-CMV-gCaMPer | Henderson et al., 2015 | RRID:Addgene #65227 |