| Antibody |
Anti-tRFP (rabbit polyclonal) |
Evrogen |
RRID:AB_2571743
|
IF(1:200) |
| Antibody |
Anti-GFP (chicken polyclonal) |
AvesLab |
RRID:AB_10000240
|
IF(1:800) |
| Antibody |
Anti-N-cadherin (rabbit polyclonal) |
Abcam |
RRID:AB_444317
|
IF(1:250) |
| Antibody |
Anti-p-myosin (rabbit polyclonal) |
Abcam |
RRID:AB_303094
|
IF(1:200) |
| Antibody |
Anti-Desmin (rabbit polyclonal) |
Sigma |
RRID:AB_476910
|
IF(1:100) |
| Antibody |
Anti-α-catenin epitope α-18 (rat monoclonal) |
Gift from Prof. Akira Nagafuchi |
|
IF(1:300) |
| Antibody |
Anti-Alcama (mouse monoclonal) |
DSHB |
RRID:AB_531904
|
IF(1:50) |
| Antibody |
Alexa Fluor 488 Goat anti Chicken IgG (H + L) |
Thermo Fisher Scientific |
RRID:AB_142924
|
IF(1:500) |
| Antibody |
Alexa Fluor 647 Goat anti Rabbit IgG (H + L) |
Thermo Fisher Scientific |
RRID:AB_141663
|
IF(1:500) |
| Antibody |
Alexa Fluor 647 Goat anti Rat IgG (H + L) |
Thermo Fisher Scientific |
RRID:AB_141778
|
IF(1:500) |
| Antibody |
Alexa Fluor 568 Goat anti Rabbit IgG (H + L) |
Thermo Fisher Scientific |
RRID:AB_2534123
|
IF(1:500) |
| Antibody |
Alexa Fluor 568 Goat anti Rat IgG (H + L) |
Thermo Fisher Scientific |
RRID:AB_2534121
|
IF(1:500) |
| Chemical compound, drug |
Agarose, low gelling temperature |
Sigma |
Cat# A9414-25g |
|
| Chemical compound, drug |
Bovine serum albumin |
Sigma |
Cat# A-9418 |
|
| Chemical compound, drug |
Chloroform |
Merck |
Cat# 102445 |
|
| Other |
DAPI |
Sigma |
Cat# D954 |
(1 µg/mL) |
| Chemical compound, drug |
Dimethyl sulfoxide (DMSO) |
Sigma |
Cat# D8418 |
|
| Chemical compound, drug |
DMEM(1X)+Glutamax |
Thermo Fisher Scientific |
Cat# 31966-021 |
|
| Chemical compound, drug |
DyNAmo ColorFlash SYBR Green qPCR Mix |
Thermo Fisher Scientific |
Cat# F416S |
|
| Chemical compound, drug |
Ethanol, undenatured, absolute |
Serva |
Cat# 11093.01 |
|
| Chemical compound, drug |
FBS superior |
Biochrom |
Cat# S0615 |
|
| Chemical compound, drug |
Glycine |
Sigma |
Cat# 50046 |
|
| Chemical compound, drug |
2-Propanol |
Roth |
Cat# 6752.4 |
|
| Chemical compound, drug |
Lipofectamine 3000 Transfection Reagent |
Thermo Fisher Scientific |
L3000001 |
|
| Chemical compound, drug |
Methanol |
Roth |
Cat# 4627.5 |
|
| Chemical compound, drug |
Normal Goat Serum |
Thermo Fisher Scientific |
Cat# 16210072 |
|
| Chemical compound, drug |
Paraformaldehyde |
Sigma |
Cat# P6148 |
|
| Chemical compound, drug |
Phosphate buffered saline (PBS) |
Sigma |
Cat# P4417 |
|
| Recombinant DNA reagent |
pT3TS-nCas9n (plasmid) |
Addgene |
Cat# 46757 |
|
| Recombinant DNA reagent |
pCS2z vector (plasmid) |
Addgene |
Cat# 62214 |
|
| Recombinant DNA reagent |
pCMV-Tol2 (plasmid) |
Addgene |
Cat# 31823 |
|
| Recombinant DNA reagent |
pGl4.14-luc; SV40:hRLuc (plasmid) |
Bensimon-Brito et al., 2020 |
|
|
| Chemical compound, drug |
Sodium citrate monobasic |
Sigma |
Cat# 71497-1KG |
|
| Chemical compound, drug |
Triton X-100 |
Sigma |
Cat# X-100 |
|
| Chemical compound, drug |
TRIzol Reagent |
Thermo Fisher Scientific |
Cat# 15596026 |
|
| Chemical compound, drug |
Tween 20 |
Sigma |
Cat# P1379 |
|
| Commercial assay or kit |
Dual-Luciferase Reporter Assay System |
Promega |
Cat# E1910 |
|
| Commercial assay or kit |
In Situ Cell Death Detection Kit, Fluorescein |
Roche |
11684795910 |
|
| Commercial assay or kit |
Maxima First Strand cDNA kit |
Thermo Fisher Scientific |
Cat# K1641 |
|
| Commercial assay or kit |
MegaShortScript T7 Transcription Kit |
Thermo Fisher Scientific |
Cat# AM1354 |
|
| Commercial assay or kit |
MegaScript T3 Transcription Kit |
Thermo Fisher Scientific |
Cat# AM1348 |
|
| Commercial assay or kit |
mMESSAGE mMACHINE T7 Transcription Kit |
Thermo Fisher Scientific |
Cat# AM1344 |
|
| Commercial assay or kit |
mMESSAGE mMACHINE T3 Transcription Kit |
Thermo Fisher Scientific |
Cat# AM1348 |
|
| Commercial assay or kit |
RNA Clean and Concentrator Kit |
Zymo Research |
Cat# R1013 |
|
| Cell line (Homo sapiens) |
HEK-293T |
ATCC |
Cat# CRL-3216 |
RRID:CVCL_0063
|
| Strain, strain background (Danio rerio) |
Tg(myl7:BFP- CAAX)bns193 |
Guerra et al., 2018 |
ZFIN:bns193 |
|
| Strain, strain background (Danio rerio) |
Tg(myl7:H2B-EGFP)zf521Tg |
Mickoleit et al., 2014 |
ZFIN:zf521Tg |
|
| Strain, strain background (Danio rerio) |
Tg(myl7:mVenus-gmnn)ncv43Tg |
Jiménez-Amilburu et al., 2016 |
ZFIN:ncv43Tg |
|
| Strain, strain background (Danio rerio) |
Tg(−0.2myl7:snai1b-p2a-GFP) bns555 |
This paper |
ZFIN:bns555 |
|
| Strain, strain background (Danio rerio) |
Tg(−0.2myl7:EGFP-podocalyxin) bns10 |
Jiménez-Amilburu et al., 2016 |
ZFIN:bns10 |
|
| Strain, strain background (Danio rerio) |
Tg(fli1a:Gal4)ubs4 |
Zygmunt et al., 2011 |
ZFIN:ubs4 |
|
| Strain, strain background (Danio rerio) |
Tg(UAS:snai1b-p2a-GFP) bns442 |
This paper |
ZFIN:bns442 |
|
| Strain, strain background (Danio rerio) |
Tg(myl7:EGFP-Hsa.HRAS)s883Tg |
D'Amico et al., 2007 |
ZFIN:s883Tg |
|
| Strain, strain background (Danio rerio) |
snai1bbn351 mutant |
This paper |
ZFIN:bns351 |
|
| Sequence-based reagent |
qPCR |
This paper |
Table S1 |
|
| Sequence-based reagent |
Genotyping |
This paper |
Table S1 |
|
| Sequence-based reagent |
PCR |
This paper |
Table S1 |
|
| Software, algorithm |
FiJi ImageJ 1.53 c |
Schindelin et al., 2012 |
RRID:SCR_002285
|
|
| Software, algorithm |
GraphPad Prism 6 |
GraphPad |
RRID:SCR_002798
|
|
| Software, algorithm |
Imaris, version 8.4.0 |
Bitplane |
RRID:SCR_007370
|
|
| Software, algorithm |
Zen Digital Imaging |
Carl Zeiss Microscopy |
RRID:SCR_013672
|
|
