Figure 7.

BGM inhibits tumor growth of melanoma in vivo. (A–D) The effect of BGM on tumor growth of melanoma in vivo was analyzed by nude mice tumorigenicity assay. The WM239 cells were injected into the nude mice (n = 6). The mice in the BGM group were intraperitoneally treated with BGM (10 mg/kg) (A) Representative images of dissected tumors from nude mice were presented. (B) The average tumor weight was calculated and shown. (C) The average tumor volume was calculated and shown. (D) The expression levels of Ki-67 of the tumor tissues were measured by immunohistochemical staining. (E) The expression of Cyclin D1 was measured by Western blot analysis in the tumor tissues of the mice. The results of Western blot analysis were quantified by ImageJ software. Data are presented as mean ± SEM. Statistic significant differences were indicated: ** P < 0.01.