Table 3.

Factors affecting sNfL levels

Variable	Univariate		Multivariate
	Estimate (95% CI)	p	Estimate (95% CI)	p
Time of measurement (days)	− 0.014 (− 0.015, − 0.012)	< 0.001	− 0.014 (− 0.014, − 0.012)	< 0.001
Poor motor outcome or death at 2 years	0.34 (0.16, 0.53)	0.001	0.28 (0.11, 0.45)	0.004
Infarction	0.22 (0.04, 0.40)	0.02	− 0.08 (− 0.28, 0.12)	0.44
Gestational age (weeks)	− 0.06 (− 0.10, − 0.01)	0.02	− 0.06 (− 0.10, − 0.02)	0.007
PIVH grade (average)	0.20 (0.06, 0.34)	0.008	0.11 (− 0.04, 0.26)	0.16
Died	0.37 (0.10, 0.65)	0.009	− 0.23 (− 0.48, 0.03)	0.09

Estimates from fitting univariate models (left) with dependent variable log₁₀ sNfL level at time (in days) of the measurements (IVH_blood, IVH-plus_blood, EVD_blood, EVD-plus_blood, term_blood); estimates from fitting the fully adjusted multivariate model (right) with random intercept and slope effects for the trajectory of each patient over time (48 patients, 153 measurements); variables with a significant effect in univariate models (p < 0.1) were included in the multivariate model. Included are all patients and all measurements. Non significant at the 5% level in univariate models: sex, post-hemorrhagic hydrocephalus, AIS amnion infection syndrome, neonatal infection, shunt infection, Cesarean section, antenatal steroids, postnatal steroids, mother’s age, SES socio-economic standard, Apgar; birth weight had a significant effect (p = 0.03), but was colinear with gestational age and, therefore, not included in the analysis. First-level interaction between time and primary endpoint was non significant at the 5% level