Table 2.
Name | Strain + Reference | Fluorophores | Resolution | Suitability | Figures | |
---|---|---|---|---|---|---|
R26-mTmG x R26-CreERT2 |
B6.129(Cg)-Gt(ROSA)26Sor tm4(ACTB-tdTomato,-EGFP)Luo /J 36271253340https://www.jax.org/strain/007676 |
constitutive membrane-tdTomato is switched to membrane-EGFP upon Cre-recombination |
- single cells - ductal trees |
- Local recombination allows the tracking of single cells - Strong fluorescence intensity allows imaging of deeper tissue layers |
- Recombination of cells requires tamoxifen injection which may potentially intefere with cellular processes in the mammary gland |
- single cell tracking: Fig. 1, 2, 3 - tracking of ductal trees: Fig. 7 |
R26-FUCCI2 |
RIKEN CLST CDB0203T 17830853340http://www2.clst.riken.jp/arg/reporter_mice.htmlhttps://dev.biologists.org/content/140/1/237 |
cell cycle driven expression of mCherryhCdt1(30/120) & mVenus-hGem(1/110) | - single cells | - Low mVenus intensity limits imaging to small ductal areas close to the tissue surface | - single cell tracking: Fig. 4 | |
CAG-KikGR-1 |
RIKEN CLST CDB0201T-1 17830853340http://www2.clst.riken.jp/arg/reporter_mice.htmlhttps://science.sciencemag.org/content/316/5825/719 |
constitutive KikGR expression. Green to Red photoconversion with 405 nm light |
- single cells - ductal segments |
- Local photoconversion allows to follow the same groups of cells over ~7 days - Moderate fluorescence intensity allows to capture larger ductal segments |
- single cell tracking: Fig. 5 |