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. 2021 May 2;142(1):159–177. doi: 10.1007/s00401-021-02317-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — W-Tau antibody validation and protein expression in human brain. a Immunoprecipitation assay of HEK239T cells overexpressing W-T30 using W-Tau (Abyntek) or total Tau (NOVUSBIO, NB100-82247) antibodies and Western blot detection of immunoprecipitated Tau using 7.51 antibody. b Immunoprecipitation of the same cells overexpressing W-T30 and control untransfected cells using W-Tau antibody and detection of immunoprecipitated Tau using total Tau (Tau 7.51) antibody. c HEK293T cells were transfected with eukaryotic expression vectors empty or encoding different MAPT isoforms (T42, T30, W-T42, W-T30, ET-42 and ET-T30). Representative Western Blot for Tau 7.51 and W-Tau antibodies proving W-Tau specificity. d Western blot detection of W-Tau in frontal lateral cortex and hippocampus of one human subject that show bands at 50 kDa (W-T42), 38 KDa (W-T30), and 31 KDa (W-Tau truncated). e Representative Western blot of frontal lateral cortex and hippocampus samples of the same three human subjects (a, b, c) showing the presence of W-Tau and total Tau (Tau5)