Table 3.
50 mL Blood transfusions, days | Conditioning | Dogs (n) | Graft rejection (%) |
---|---|---|---|
None[26,27] | 9.2Gy TBI | 62 | 2 |
Marrow donor day-24, day-17, day-10 [4,26] | 9.2Gy TBI | 27 | 100 |
Marrow donor day-24, day-17, day-10 | 9.2Gy TBI with 211At-anti-CD45 mAb | 7 | 14 |
In our well-established canine model, following 9.2Gy TBI conditioning, only 2% (1/62) of un-transfused dogs rejected the marrow grafts from their DLA-identical littermate donors. However, 100% (27/27) of the dogs pretreated with three unirradiated transfusions from their respective DLA-identical marrow donors rejected their grafts. 211At-anti-CD45 mAb in combination with 9.2Gy TBI is successful in abrogating graft. Rejection was only 14% (1/7) of dogs in the pre-sensitization model. n: number; TBI: total body irradiation.