Climbing fibers (CFs) pathology in the essential tremor (ET) cerebellar cortex. (A–C) Representative images of CFs, labeled by cocaine and amphetamine-regulated transcript (CART) (brown), and Purkinje cells (PCs), labeled by calbindin (red), in a control (A) and three ET cases (B, C). Arrows, CART-labeled CF crossings. Dashed lines, the pial surface of the cerebellar cortex. Scale bar: 25 μm. (D, E) ET cases have CFs crossing more PC somas and PC dendrites in comparison with control cases. (F, G) The number of PC somas or the number of PC dendrites crossed by a CF correlates with tremor severity. Error bars represent the standard deviation.