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. 2021 Jun 21;12:3810. doi: 10.1038/s41467-021-24030-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Schematic overview of the proteasome assembly line. B Peptide profiles of the proteasome subunit-β type-7 (β7, PSMB7, UniProt ID: Q99436) in interphase. Peptides of the two assigned proteoform groups are colored in orange and blue. A zoom-in on fraction 10 to 40 is shown in log scale, including an additional semi-tryptic peptide TTIAGVVYK that represents the N-terminal tryptic peptide of the processed proteoform (green). Please note that the abundance values between the orange and blue peptides cannot be directly compared to the semi-tryptic peptide TTIAGVVYK in green because of the separate analysis platforms used. C Protein sequence plot for PSMB7. Sequence coverage and position of the detected peptides of the two assigned proteoform groups are indicated in blue and orange. Pro-peptide and chain information from UniProt are indicated as horizontal bars. The known pro-peptide cleavage site is indicated by scissors. A zoom-in representation is provided for the region around the annotated cleavage site. The semi-tryptic peptide from B is highlighted in green. D Schematic overview of the NUP98 and NUP96 proteoform biogenesis from the same mRNA and precursor protein (UniProt ID: P52948). E Peptide profiles of the Nup98 gene product in interphase (left) and mitosis (right). Peptides of the two assigned proteoform groups are colored in orange and blue. F Protein sequence plot for the Nup98 gene product. Sequence coverage and position of the detected peptides of the two assigned proteoform groups are indicated in blue and orange. Chain information and alternative sequence isoforms from UniProt are indicated as horizontal bars. The known autocatalytic cleavage site of NUP98/96 is shown as an arrow in a zoom-in on the relevant sequence region.