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. 2021 Jun 21;11:12993. doi: 10.1038/s41598-021-92406-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of individual lipid trajectories in mdx and WT mice. (A) Scatter plot showing the enrichment in low P values in the observed distribution compared to the expected one. (B) Heatmap showing the abundance of 29 lipids with adjusted P.global < 0.001. Hierarchical clustering shows complete separation of dystrophic and WT animals. (C) Circular plots showing the correlation across all lipids and the 29 selected lipids in both WT and mdx mice. Despite the difference in abundance, the correlation across the lipids was maintained. (D) Plot showing the number of lipids significantly dysregulated at each time point in mdx mice. At 12 weeks of age the highest number differences was recorded. (E–G) Line plots showing the differences for 3 specific lipids at all time points.