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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: J Hepatol. 2021 Feb 9;75(1):34–45. doi: 10.1016/j.jhep.2021.01.043

Fig. 3. Magnesium distribution after specific silencing of Cnnm4.

Fig. 3.

(A) Magnesium in serum from mice fed a 0.1% MCDD or CD-HFD with specific Cnnm4 silencing (siCnnm4) compared with non-treated (siCtrl) mice. (B) Biochemical structure of non-targeted MagS and MagS-TPP probes. (C) Micrographs and relative intracellular magnesium levels and (D) extracellular magnesium levels in primary hepatocytes treated with an siRNA against Cnnm4 (siCnnm4). Scale bar corresponds to 100 μm. (E) Intracellular magnesium and (F) extracellular magnesium levels in primary hepatocytes treated with siCnnm1-3. (G) Schematic representation of CNNM4-dependent magnesium fluctuations in liver and in circulation **p <0.01 vs. SC diet; #p <0.05, and ##p <0.01 vs. 0.1% MCDD + siCtrl/CD-HFD + siCtrl/siCtrl. CD-HFD, choline-deficient high-fat diet; CNNM4, cyclin M4; MagS, magnesium-specific; MagS-TPP, mitochondrion-targeted magnesium-specific; MCDD, methionine and choline deficient diet; siRNA, small-interfering RNA.