Table 3.
BLAST comparisona | ||
---|---|---|
Chlamydomonas reinhardtii | Homo sapiensb | Potential related/associated disease in humans |
Actin/DII4/IDA5 | ACTG1 (0.0)c, d | Various |
Centrin/DLE2/VFL2 | CETN2 (2e-55) | - |
FAP120/DII7 | N/Ae | - |
IC140/DIC3/IDA7 | WDR63/DNAI3 (6e-55) | Occipital encephalocele (Hofmeister et al., 2018) |
IC138/DIC4/BOP5 | WDR78/DNAI4 (2e-68) | - |
IC97/DII6/FAP94 | CASC1/CFAP94 (2e-11) | Pulmonary carcinoma (Galbiati et al., 2006) |
LC7a/DLR1/ODA15 | DYNLRB2 (1e-40) | - |
LC7b/DLR2 | DYNLRB2 (3e-20) | - |
LC8/DLL1/FLA14 | DYNLL2 (2e-56) | - |
MOT7 | TEX47 (6e-14) | Hypothyroidism (Eriksson et al., 2012) |
NAP/DII5 | N/Af | - |
p28/DII1/IDA4 | DNALI1 (1e-93) | - |
p38/DII2/FAP146 | ZMYND12 (1e-19) | - |
p44/DII3 | TTC29 (2e-13) | Asthenoteratospermia/Asthenozoospermia (Liu et al., 2019; Lorès et al., 2019) |
TCTEX1/DLT3 | DYNLT1 (2e-42) | - |
TCTEX2b/DLT4 | TCTEX1D2/DYNLT2B (5e-35) | Jeune asphyxiating thoracic dystrophy (Schmidts et al., 2015) |
For the presence/absence of the potential homologous DHCs of Chlamydomonas IDAs in humans, see the previous papers (Hom et al., 2011; Kollmar, 2016; Morris et al., 2006). For potential links between human IDA DHCs and diseases, see Section 2-1 in the main text.
Blast search using the Chlamydomonas sequences as queries against the Ensembl genome browser 102 human database (http://www.ensembl.org/Homo_sapiens/Info/Index).
Gene names and BLAST e-Values are shown in the columns.
Letters in bold in human proteins mean that the reciprocal BLAST search against Chlamydomonas Phytozome genome database (v5.5) identified the corresponding dynein subunit as the best hit in Chlamydomonas reinhardtii.
Because of the presence of ANK repeats, the exact human orthologue(s) of FAP120 in humans is hard to determine (Ikeda et al., 2009).
From the detailed phylogenetic analyses, homologues of NAP/DII5 are only present in algae phylogenetically close to Chlamydomonas (Kato-Minoura et al., 2003; Kato-Minoura et al., 1998).