Skip to main content
. Author manuscript; available in PMC: 2022 Jun 16.
Published in final edited form as: Neuron. 2021 May 24;109(12):1963–1978.e5. doi: 10.1016/j.neuron.2021.04.021

Figure 3. Rapsn LLPS into liquid-like compartments in myotubes and in muscles.

Figure 3.

(A) Fusion of small spontaneous Rapsn-EGFP aggregates into large, continuous clusters in myotubes. Arrow, cluster during fusion.

(B) Dynamic property of Rapsn-EGFP protein within spontaneous clusters.

(C) Quantification of fluorescence recovery in (B).

(D) Fusion of agrin-induced Rapsn-EGFP clusters in myotubes.

(E-H) Dynamic exchange of Rapsn-EGFP between cluster and surrounding milieu, and within cluster in myotubes. (E) FRAP analysis of a agrin-induced Rapsn-EGFP cluster, and (F) quantification of fluorescence recovery; (G) FRAP analysis of a part of cluster and (H) quantification of fluorescence recovery.

(I, J) Dynamic property of Rapsn-EGFP in living muscles.

Data was shown as mean ± SEM; n = or > 3.