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. 2021 Jun 8;15:605030. doi: 10.3389/fncel.2021.605030

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Copyright © 2021 Iwasa, Matsui, Iguchi, Kinugawa, Morikawa, Sakaguchi, Shiota, Kobashigawa, Nakanishi, Matsubayashi, Nagata, Kikuchi, Tanaka, Eura, Kiriyama, Izumi, Saito, Kataoka, Saito, Kimura, Wanaka, Nishimura, Mori and Sugie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) qPCR analysis of cerebral organoids [control vs. OGD/R condition]. Gene expression analysis of AHSG, TTR, and FGG on non-treated organoids (Ctrl) vs. organoids after OGD/R (OGD/R). The P-values were 0.0138 (AHSG), 0.0185 (TTR), and 0.0104 (FGG). *P < 0.05 vs. ctrl. (B) Tissue-specific gene expression levels using GTEx database. AHSG, TTR, and FGG. The Y-axis indicates transcripts per million (TPM). (C) After OGD/R, expression level of AHSG, validated using hepatocellular carcinoma (HepG2), telomerase reverse transcriptase immortalized fibroblasts (hTERT-BJ), glioblastoma (U251MG), and murine microglia (BV2). TTR and FGG were not detected in BV2 cells and TTR was not detected in hTERT-BJ.