Fig. 3. Migration and relative abundance of canonical and putatively associated components of respiratory chain complexes III and IV.

An abundance of 1 (red) represents the highest iBAQ value for a given protein between both samples. a Heatmap showing comigration of canonical CIII components as well as putative novel components migrating at an M_r^app. of ~730 kDa in ABS parasites (left) and gametocytes (right) respectively. b Heatmap showing comigration of canonical CIV components as well as putative novel components migrating at an M_r^app. of ~570 kDa as well as relative abundance in ABS parasites (left) and gametocytes (right). c For detailed analysis of higher-order assemblies, intensity values at M_r^app. >750 kDa were renormalized and visualized in a lineplot. Different putative supercomplexes were observed in ABS parasites and gametocytes, denoted with lettering and described in the graph inlet. App. approximate molecular mass based on migration profile, Exp. expected molecular mass based on composition observed in this study, SC supercomplex, CIII₂ obligatory CIII dimer, CIII₄ association of two CIII dimers, CIV CIV monomer, CIV₂ CIV dimer.