Skip to main content
. 2021 Jun 21;12:3820. doi: 10.1038/s41467-021-23919-x

Table 1.

Divergent composition of the respiratory chain complexes in Plasmodium falciparum.

Name Gene ID Mol. Massa Coverageb MS/MS countb MFSc ToxoGeneIDd Tfite LOPITf
CII SDHA PF3D7_1034400 70.7 29.8 219 −2.83 TGME49_215590 −3.96 mito-soluble
SDHB PF3D7_1212800 378 25.2 53 −0.41 TGME49_215280 −2.00 mito-memb
PfSDHC PF3D7_1448900 8.9 30.7 31 −2.87 TGME49_227920 −2.42 /
C2AP1 PF3D7_0808450 10.6 20 10 / / / /
C2AP2 PF3D7_1322800 14.0 25.2 30 0 TGME49_252630 −1.45 mito-memb
C2AP3 PF3D7_0109950 19.5 35.9 44 / TGME49_315930 −3.84 mito-memb
C2AP4 PF3D7_1346600 26.7 14 21 −0.26 TGME49_306650 −1.80 mito-memb
Monomer 188
Trimer 564
CIII Cytb mtDNA 43.4 16.0 143 / / / /
Cytc1 PF3D7_1462700 46.1 43.1 254 / TGME49_246540 −4.36 mito-memb
Rieske PF3D7_1439400 41.0 59.7 646 −2.58 TGME49_320220 −5.76 mito-memb
QCR6 PF3D7_1426900 11.0 / / −2.49 TGME49_320140 −3.69 mito-memb
QCR7 PF3D7_1012300 23.0 78.1 355 −2.62 TGME49_288750 −4.04 mito-memb
QCR8 PF3D7_0306000 17.0 38.7 95 −2.85 TGME49_227910 −3.20 mito-memb
QCR9 PF3D7_0622600 11.7 53.1 118 −3.1 TGME49_201880 −3.68 mito-memb
MPPalpha PF3D7_0933600 55.7 54.5 928 −2.98 TGME49_202680 −4.30 mito-memb
MPPbeta PF3D7_0523100 61.8 72.3 1345 −2.75 TGME49_236210 −4.74 mito-memb
C3AP1 PF3D7_0722700 9.5 32.9 55 0 TGME49_214250 −3.94 mito-memb/outlier
C3AP2 PF3D7_1326000 19.3 56.4 218 −2.74 TGME49_207170 −3.44 mito-memb
C3AP3 PF3D7_0817800 6.2 38.9 8 0 TGME49_312940 −2.27 /
Dimer 690
CIV COX1 mtDNA 57.0 7.0 41 / / / /
COX2a PF3D7_1361700 27.3 44.3 71 −2.84 TGME49_226590 −3.80 mito-memb
COX2b PF3D7_1430900 19.8 52.9 96 −2.53 TGME49_310470 −4.18 /
COX3 mtDNA 32.3 6.5 14 / / / /
PfCOX4 PF3D7_0708700 27.6 26.8 200 −2.42 TGME49_262640 −3.49 mito-memb
COX5b PF3D7_0927800 32.4 53.8 187 0 TGME49_209260 −3.07 mito-memb
PfCOX6a PF3D7_1465000 33.3 37.4 282 −2.15 TGME49_264040 −2.54 mito-memb
COX6b PF3D7_0928000 12.2 53.4 14 0 TGME49_200310 −0.05 mito-memb
PfCOX6c PF3D7_0306500 36.4 44.1 294 −2.69 TGME49_229920 −3.84 mito-memb
PfNDUFA4 PF3D7_1439600 22.6 63.9 247 −2.71 TGME49_306670 −3.68 mito-memb
ApiCOX13 PF3D7_1022900 13.9 63.6 92 −2.01 TGME49_254030 −4.26 mito-memb
ApiCOX14 PF3D7_1339400 17.8 40.5 96 −2.42 TGME49_242840 −3.58 mito-memb/soluble
ApiCOX16 PF3D7_1345300 10.2 63.1 65 −3.07 TGME49_265370 0.65 mito-memb
ApiCOX18 PF3D7_0523300 16.0 56.2 42 −2.92 TGME49_221510 −3.28 mito-memb
ApiCOX19 PF3D7_1402200 22.1 39.4 78 −2.67 TGME49_247770 −2.61 mito-memb
ApiCOX24 PF3D7_1362000 23.8 39.2 114 −2.19 TGME49_286530 −2.82 mito-memb
ApiCOX30 PF3D7_0915700 22.4 60.8 192 −2.96 TGME49_297810 −3.64 mito-memb
C4AP1 PF3D7_1125600 10.0 45.8 44 0 TGME49_316255 0.19 mito-memb/outlier
C4AP2 PF3D7_1025800 8.2 32.8 48 −3.46 TGME49_263630 0.11 mito-memb
C4AP3 PF3D7_1003100 10.7 35.1 63 0 TGME49_200310 −0.05 mito-memb
C4AP4 PF3D7_0608400 12.7 17.9 12 −2.26 TGME49_312160 −1.29 mito-memb
C4AP5 PF3D7_0809250 7.9 47.8 19 / TGME49_225555 −1.20 mito-memb
Monomer 476
CV OSCP PF3D7_1310000 30.2 15.8 4 −3.12 TGME49_284540 −3.94 mito-memb
ATPα PF3D7_0217100 61.8 49.5 903 −2.78 TGME49_204400 −3.84 mito-memb
ATPβ PF3D7_1235700 58.4 82.1 1741 −2.51 TGME49_261950 −4.84 mito-memb
ATPγ PF3D7_1311300 35.8 12.5 12 −3.03 TGME49_231910 −3.94 mito-memb
ATPd PF3D7_0311800 73.5 9.9 8 −3.08 TGME49_268830 −2.02 mito-memb
ATPb PF3D7_1125100 59.3 17.7 15 −3.05 TGME49_231410 −5.37 mito-memb
ATPk PF3D7_0107400 18.8 65.4 29 −2.43 TGME49_260180 −4.07 mito-memb
ATP i/j PF3D7_1360000 21.6 22 6 −3.11 TGME49_290030 −3.88 mito-memb
ATPδ PF3D7_1147700 17.6 71.2 19 −1.7 TGME49_226000 −4.57 mito-memb
ATPε PF3D7_0715500 8.5 66.2 12 −1.82 TGME49_314820 −3.21 mito-memb
ATPag PF3D7_0719100 21.3 / / −3.6 TGME49_310360 −4.49 /
ATPcg PF3D7_0705900 18.6 / / −3.39 TGME49_249720 −2.98 /
ATP8 PF3D7_0934300 16.9 21.4 8 −2.98 TGME49_208440 −3.54 mito-memb
ATPTG2 PF3D7_0611300 33.5 23.2 15 −3.24 TGME49_282180 −2.46 mito-memb
ATPTG3 PF3D7_0815400 18.8 28.1 8 −2.76 TGME49_218940 −3.92 mito-memb
ATPTG4 PF3D7_0905000 34.0 21.7 11 −3.29 TGME49_201800 −4.01 mito-memb
ATPTG6 PF3D7_1142800 35.5 22.2 13 −2.82 TGME49_223040 −4.49 mito-memb
ATPTG9 PF3D7_1417900 16.8 18.8 16 −2.4 TGME49_285510 −1.87 mito-memb
ATPTG10 PF3D7_1024300 15.6 11.5 3 −3.27 TGME49_214930 −1.37 mito-memb
ATPTG12 PF3D7_0620100 15.5 23.8 2 −3.77 TGME49_245450 −2.95 mito-memb
ATPTG13 PF3D7_1303000 13.8 24.8 5 −2.21 TGME49_225730 −3.65 mito-memb
ATPTG15 PF3D7_0825400 14.2 28.6 7 −2.39 TGME49_247410 −3.90 mito-memb
ATPTG17 PF3D7_0306600 9.9 15.3 9 −3.03 TGME49_310180 −3.4 mito-memb
Monomer 1076
Dimer 2152

aIn kDa; estimates based on predicted amino acid composition, no post-translational modifications, cleavage events or lipid association were assumed. For complex mass estimation standard stoichiometry for conserved components (10× ATPc per CV monomer) and 1:1 stoichiometry for novel components was assumed.

bBased on all samples.

cMean fitness scores as an indicator of P. falciparum gene essentiality97.

dAssigned based on homology searches using HHpred53 at default settings against T. gondii proteome (E < 0.05).

eTfit scores as an indicator of T. gondii gene essentiality98.

fLocalization estimates of T. gondii proteins99.

gATPa and ATPc were not detected by MS but given their evolutionary conserved nature are expected to form part of the complex.