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. 2021 Jun 21;12:3803. doi: 10.1038/s41467-021-23501-5

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–d KYSE180 cells with or without METTL3 depletion (a), or with reconstituted expression of an RNAi-resistant (r) METTL3 (c) were analysed by immunoblotting analyses. The cell proliferation (a, c) and colony formation (b, d) assays were performed. Data represent means ± SD of triplicate samples. a ***p = 0.0004 (top), 0.0002 (bottom). b ***p = 0. 0004 (left), 0.0004 (right) (two-tailed t-test). c ***p = 4.72E − 05. d ***p = 0.0007. Two-tailed t-test; ns, not significant. e, f KYSE450 cells expressing the METTL3 expression-inducible Tet-on lentiviral vector were treated with or without the indicated dosages of tetracycline, followed by immunoblotting analyses. The cell proliferation (e) and colony formation (f) assays were performed. Data represent means ± SD of triplicate samples. e ***p = 6.37E − 04 (top), ***p = 9.58E − 04 (bottom). f ***p = 9.24E − 04 (left), **p = 0.0095 (right). Two-tailed t-test. g, h KYSE450 cells with or without expressing WT METTL3 or an inactive METTL3 mutant were analysed by immunoblotting analyses. The cell proliferation (g) and colony formation (h) assays were performed. Data represent means ± SD of triplicate samples. ***p = 0.0004 (g), ***p = 0.0003 (h). Two-tailed t-test; ns, not significant. i–k KYSE180 cells with or without METTL3 depletion were subcutaneously injected into the mice (n = 6). Six weeks later, tumour sizes (i), volumes (j) and weight (k) were measured. Scale bar, 1 cm. Data represent means ± SD of six mice per group. ***p = 2.58E − 06 (j), *p = 0.0138 (k) (two-tailed t-test). l–n KYSE180 cells with or without expressing WT METTL3 or an inactive METTL3 mutant were subcutaneously injected into the mice (n = 6). Five weeks later, tumour sizes (l), volumes (m) and weight (n) were measured. Scale bar, 1 cm. Data represent means ± SD of six mice per group. **p = 0.0015 (m), 0.0013 (n) (two-tailed t-test). ns, not significant. Source data are provided as a Source Data file.