Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 21;12:3803. doi: 10.1038/s41467-021-23501-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a RIP analyses of KYSE180 cells were performed with an anti-YTHDF1 or anti-YTHDF2, or anti-YTHDF3 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. ***p = 0.0006 (left), **p = 0.0092 (right) based on two-tailed Student’s t-test. b KYSE180 cells were transfected with or without a vector expressing METTL3 shRNA. RIP analyses were performed with an anti-YTHDF2 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. ***p = 1.85E − 05 (left), **p = 0.0023 (middle) and 0.0056 (right) based on two-tailed Student’s t-test. c KYSE450 cells were transfected with or without a vector expressing YTHDF2 shRNA. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. ***p = 0.0006, 0.0009, 5.58E − 05 and 0.0007 (left to right) based on two-tailed Student’s t-test. d KYSE450 cells were transfected with or without YTHDF2 shRNA. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. e KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. ***p = 9.33E − 04 (left) and 2.99E − 05 (right), *p = 0.031 based on two-tailed Student’s t-test. f KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. g KYSE180 cells expressing luciferase reporter genes fused with or without the wild-type (WT) or mutated m6A nucleotides from APC genes were transfected with or without a vector expressing YTHDF2 shRNA. The relative luciferase activity after normalization to the shControl group is shown. Data represent the means ± SD of triplicate samples. ***p = 3.94E − 06 based on two-tailed Student’s t-test. ns, not significant. Source data are provided as a Source Data file.