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. 2021 Jun 21;11:12973. doi: 10.1038/s41598-021-92332-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of hits of EIF5A and related genes and genes involved in UDP-glycosylation pathway. THP-1 iCas9 cells were transduced with guides targeting indicated genes or with non-targeting control (NTC) and Cas9 expression was induced with dox. On day 12 after gene knockout, S. aureus, E. coli and zymosan A particles labeled with pHrodo red were added and phagocytosis was measured 1 h later using flow cytometry. Depicted is the mean phagocytosis index of live Thy1.1⁺ cells of 2–5 replicates ± SEM. The phagocytosis index is the mean fluorescence intensity normalized to non-transduced THP-1 cells. To test statistical significance, a one-way ANOVA corrected for multiple comparison according to Dunnett was performed. Ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.