Table 1.
Differentially expressed proteins in maize seedlings to salt stress (100mM NaCl).
| Number | Gene | Annotation | Percent coverage | No.of unique peptides | Mean ratioa | Up/downb |
|---|---|---|---|---|---|---|
| Translation, ribosomal structure and biogenesis | ||||||
| 1 | sp|P08530|RR8_MAIZE | Ribosomal protein S8 | 33.09% | 4 | 0.813 | ↓ |
| 2 | sp|P17788|RK2_MAIZE | Ribosomal protein L2 | 22.34% | 4 | 0.833 | ↓ |
| 3 | tr|B6U1J2|B6U1J2_MAIZE | Ribosomal protein L11 | 19.82% | 3 | 1.372 | ↑ |
| 4 | tr|B4FVB2|B4FVB2_MAIZE | Translation elongation factor EF-Tu, a GTPase | 28.32% | 2 | 1.391 | ↑ |
| 5 | tr|A0A096U6X9|A0A096U6X9_MAIZE | Translation elongation factor EF-G, a GTPase | 29.39% | 2 | 0.706 | ↓ |
| 6 | tr|K7TKZ6|K7TKZ6_MAIZE | Ribosome-binding ATPase YchF, GTP1/OBG family | 20.87% | 6 | 1.202 | ↑ |
| 7 | tr|O82108|O82108_MAIZE | Seryl-tRNA synthetase | 6.35% | 3 | 1.457 | |
| 8 | tr|K7TUG6|K7TUG6_MAIZE | Protein chain release factor A | 14.56% | 4 | 0.764 | ↓ |
| Post-translational modification, protein turnover, chaperones | ||||||
| 9 | tr|B6UFB3|B6UFB3_MAIZE | Molecular chaperone DnaK (HSP70) | 33.71% | 3 | 0.77 | ↓ |
| 10 | tr|A0A096RH43|A0A096RH43_MAIZE | Chaperonin GroEL (HSP60 family) | 58.43% | 1 | 0.699 | ↓ |
| 11 | tr|A0A096PXR7|A0A096PXR7_MAIZE | Peroxiredoxin | 46.86% | 8 | 1.378 | ↑ |
| 12 | tr|B4FSF1|B4FSF1_MAIZE | ATP-dependent Zn proteases | 18.48% | 5 | 1.425 | ↑ |
| 13 | tr|B4FTV9|B4FTV9_MAIZE | ATP-dependent Zn proteases | 9.75% | 2 | 1.212 | ↑ |
| 14 | tr|B4FZZ2|B4FZZ2_MAIZE | Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family | 40.70% | 5 | 1.311 | ↑ |
| Energy production and conversion;Inorganic ion transport and metabolism | ||||||
| 15 | tr|K7URG3|K7URG3_MAIZE | Citrate synthase | 32.97% | 3 | 1.356 | ↑ |
| 16 | tr|A0A096S8W2|A0A096S8W2_MAIZE | Citrate synthase | 9.94% | 4 | 1.219 | ↑ |
| 17 | tr|Q49HD8|Q49HD8_MAIZE | 2,4-dienoyl-CoA reductase | 13.78% | 4 | 1.297 | ↑ |
| 18 | tr|B6TS21|B6TS21_MAIZE | Succinyl-CoA synthetase, beta subunit | 52.84% | 3 | 1.252 | ↑ |
| 19 | sp|P19023|ATPBM_MAIZE | FoF1-type ATP synthase, beta subunit | 48.64% | 2 | 1.627 | ↑ |
| 20 | tr|A0A096QV77|A0A096QV77_MAIZE | Inorganic pyrophosphatase | 20.90% | 1 | 0.736 | ↓ |
| Amino acid transport and metabolism | ||||||
| 21 | sp|Q43260|DHE3_MAIZE | Glutamate dehydrogenase/leucine dehydrogenase | 37.23% | 9 | 1.253 | ↑ |
| 22 | tr|A0A096TF85|A0A096TF85_MAIZE | 3-deoxy-D-arabino-heptuloso-nate 7-phosphate (DAHP) synthase, class II | 13.83% | 3 | 0.688 | ↓ |
| 23 | tr|B4F7V1|B4F7V1_MAIZE | 3-deoxy-D-arabino-heptuloso-nate 7-phosphate (DAHP) synthase | 13.06% | 3 | 1.235 | ↑ |
| 24 | tr|B4G1Z7|B4G1Z7_MAIZE | 3-dehydroquinate synthetase | 20.57% | 2 | 0.773 | ↓ |
| 25 | tr|A0A096TVA7|A0A096TVA7_MAIZE | O-acetylserine sulfhydrylase, pyridoxal phosphate- dependent | 12.68% | 5 | 2.253 | ↑ |
| 26 | tr|B6T7Q7|B6T7Q7_MAIZE | glycine/serine hydroxymethyltransferase | 18.13% | 8 | 1.242 | ↑ |
| 27 | tr|B4FAI1|B4FAI1_MAIZE | N-acetyl-gamma-glutamylphosphate reductase | 12.26% | 4 | 1.376 | ↑ |
| Carbohydrate transport and metabolism | ||||||
| 28 | sp|P08735|G3PC1_MAIZE | glyceraldehyde-3-phosphate dehydrogenase/Erythrose-4-phosphate ehydrogenase | 67.95% | 8 | 1.484 | ↑ |
| 29 | tr|B4FQW6|B4FQW6_MAIZE | glyceraldehyde-3-phosphate dehydrogenase /erythrose-4-phosphate dehydrogenase | 77.63% | 7 | 1.549 | ↑ |
| 30 | tr|A0A096R9A2|A0A096R9A2_MAIZE | Pentose-5-phosphate-3-epimer-ase | 7.38% | 1 | 1.205 | ↑ |
| General function prediction only | ||||||
| 31 | tr|B6T5H6|B6T5H6_MAIZE | Predicted oxidoreductase (related to aryl-alcohol dehydrogenase) | 34.80% | 10 | 1.339 | ↑ |
| Lipid transport and metabolism | ||||||
| 32 | tr|A0A096Q2N4|A0A096Q2N4_MAIZE | Biotin carboxylase | 4.69% | 2 | 1.27 | ↑ |
| 33 | tr|A0A096UC49|A0A096UC49_MAIZE | Acetyl-CoA acetyltransferase | 47.88% | 12 | 1.33 | ↑ |
| 34 | tr|H2BJB6|H2BJB6_MAIZE | Acyl-CoA synthetase (AMP-forming) | 19.54% | 8 | 1.202 | ↑ |
| 35 | tr|A0A096QRK2|A0A096QRK2_MAIZE | 1-deoxy-D-xylulose 5-phosphate reductoisomerase | 25.21% | 8 | 0.814 | ↓ |
| Nucleotide transport and metabolism | ||||||
| 36 | tr|A0A096S8D2|A0A096S8D2_MAIZE | Nucleoside diphosphate kinase | 42.61% | 4 | 1.723 | ↑ |
| 37 | tr|A0A096UA28|A0A096UA28_MAIZE | Nucleoside diphosphate kinase | 34.44% | 6 | 1.393 | ↑ |
| Secondary metabolites biosynthesis, transport and catabolism | ||||||
| 38 | tr|A2T1W7|A2T1W7_MAIZE | Aldo/keto reductase, related to diketogulonate reduct-ase | 23.55% | 7 | 1.515 | ↑ |
| Inorganic ion transport and metabolism | ||||||
| 39 | tr|B6SKA7|B6SKA7_MAIZE | Sulfate adenylyltransferase subunit 1 | 38.03% | 2 | 1.211 | ↑ |
| Coenzyme transport and metabolism | ||||||
| 40 | tr|B4FAD1|B4FAD1_MAIZE | S-adenosylmethionine synthetase | 57.58% | 7 | 0.709 | ↓ |
| 41 | tr|B4FSE1|B4FSE1_MAIZE | Archaeal ribulose 1,5-bisphosphate synthetase | 28.01% | 1 | 0.587 | ↓ |
| 42 | sp|Q41739|THI42_MAIZE | Archaeal ribulose 1,5-bisphosphate synthetase | 28.81% | 3 | 0.805 | ↓ |
| 43 | tr|B6SJR7|B6SJR7_MAIZE | 5,10-methylene-tetrahydrof-olate dehydrogenase | 11.65% | 3 | 1.226 | ↑ |
| 44 | tr|A0A096TKH4|A0A096TKH4_MAIZE | Delta-aminolevulinic acid dehydratase, porphobilinogen synthase | 42.25% | 14 | 0.79 | ↓ |
| Cell wall/membrane/envelope biogenesis | ||||||
| 45 | tr|B4FAG0|B4FAG0_MAIZE | Nucleoside-diphosphate-sugar epimerase | 29.71% | 3 | 1.216 | ↑ |
| 46 | tr|B4FF24|B4FF24_MAIZE | dTDP-D-glucose 4,6- dehydratase | 22.51% | 7 | 0.797 | ↓ |
| Cell cycle control, cell division, chromosome partitioning;Cell wall/membrane/envelope biogenesis;Signal transduction mechanisms | ||||||
| 47 | tr|A0A096S4K3|A0A096S4K3_MAIZE | Chromosome partitioning ATPase, Mrp family, contains Fe-S cluster | 22.51% | 7 | 0.797 | ↓ |
| 48 | tr|C0P5X1|C0P5X1_MAIZE | AAA+-type ATPase, SpoVK/Ycf46/Vps4 family | 61.36% | 15 | 0.798 | ↓ |
| Other metabolism | ||||||
| 49 | tr|A0A096QFD4|A0A096QFD4_MAIZE | 14.53% | 5 | 1.215 | ↑ | |
| 50 | tr|A0A059Q6U2|A0A059Q6U2_MAIZE | 16.76% | 12 | 0.786 | ↓ | |
| 51 | tr|A0A059Q6W8|A0A059Q6W8_MAIZE | 13.60% | 11 | 0.734 | ↓ | |
| 52 | tr|A0A096PU70|A0A096PU70_MAIZE | 14.75% | 5 | 0.824 | ↓ | |
| 53 | tr|A0A096SA34|A0A096SA34_MAIZE | 3.04% | 2 | 0.787 | ↓ | |
| 54 | tr|K7USR3|K7USR3_MAIZE | 18.63% | 6 | 0.667 | ↓ | |
| 55 | tr|C0HIU5|C0HIU5_MAIZE | 18.56% | 4 | 1.434 | ↑ | |
| 56 | tr|B6T681|B6T681_MAIZE | 56.38% | 2 | 0.634 | ↓ | |
aMean ratio corresponds to the protein reporter ion intensity originating from salt-treated protein samples(113 and 114) relative to fully control samples (115 and 116)with a 1.2 fold-changes and p<0.05.
bProteins increased in abundance(↑) or decreased in abundance(↓)