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. 2021 Jan 1;19(1):e2775. doi: 10.30498/IJB.2021.2775

Table1.

Primer details of proline biosynthesis and degradation genes (P5CS and PRODH, respectively) and a housekeeping gene (Elongation factor 1-alpha) used in this study.

Gene Primer* Tm (°C)
Elongation factor 1-alpha F: 5′ ACAAAATCCCATTCGTCCCCATC 3′ 55.2
R: 5′ ACTGGCACCGTTCCAATACCAC 3′ 57.2
P5CS F: 5′ GCTACAGCACAAGAAGCTGGAC 3′ 56
R: 5′ TCCAAAACAAGACCATCTGCCAC 3′ 55.6
PRODH F: 5′ CTGAAGACACAATCCTCCAACCC 3′ 55.2
R: 5′ CACCTCTCACCAACTTAAACCCC 3′ 55.1
*

F: forward, R: reverse.