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The specific and universal primers sequence. The underlines indicated restriction enzyme sites.
| Name | Sequence |
|---|---|
| Nb-BacF (BamHI) | 5'-ACGGGATCCACAGGTGCAGCTGCAGGAGTCTGG-3' |
| Nb-BacR (Stop-XhoI) | 5'-ACGCTCGAGTTATGAGGAGACGGTGACCTGG-3' |
| polh-Forward | 5'-AAATGATAACCATCTCGC-3' |
| PFSBC-Reverse | 5'-CCTCTACAAATGTGGTATGGC-3' |
| pUC/M13-Forward | 5'-CCCAGTCACGACGTTGTAAAACG-3' |
| pUC/M13-Reverse | 5'-AGCGGATAACAATTTCACAGG-3' |
