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. 2021 Jun 21;4:765. doi: 10.1038/s42003-021-02283-y

Fig. 3. Relative expression of genes related to hypocotyl elongation, auxin levels, and auxin transport activity in GCHXK seedlings.

Fig. 3

a–c RT-PCR expression analysis of genes related to the hypocotyl elongation of WT and GCHXK seedlings grown in 0.5 MS agar media. TUB2 (β-tubulin) was used for normalization and the expression level in the WT was set to one. Data points are mean ± SE (n = 5). d GCHXK promotes the accumulation of auxin. IAA, IAAsp, IAGlu, and OxIAA quantification in 5-day-old WT and GCHXK seedlings. Data points for are means ± SE (n = 4 and 5 for WT and GCHXK, respectively). a–d Black dots represent individual data points, and blue asterisks indicate a significant difference relative to the WT (t test, P < 0.05). e Auxin transport activity is required for hypocotyl growth of GCHXK. WT and GCHXK seedlings grown in 0.5× MS agar media containing 1 or 5 µM of NPA for 9 days. The box plots extend from the first to third quartiles and the whiskers extend from the minimum to the maximum levels. Lines within the boxes signify median values and light gray dots represent individual data points (n = 12–25). Different lower-case and capital letters indicate significant differences (Tukey’s HSD test, P < 0.05).