Figure 2.

PprSKD showed reduced survival to acute IR and reduced growth under both acute and chronic IR. (A) Survival curve of D. radiodurans PprS knockdown mutant (PprSKD), PprSKD with empty plasmid control (PprSKD_EV), PprS complementation (PprSCom) and WT at different doses of acute IR. Error bars represent standard deviation of biological triplicates and *p-value < 0.05 between the strain compared to WT by two-tailed Student’s t-test. (B) Growth of WT, PprS knockdown and complement strains, and a recA defect strain (rec30) under chronic irradiation at 57 Gy/h for 5 days. Numbers over the images indicate the dilution ratio of colonies. (C) Doubling time of biological triplicates of WT and PprS knockdown and complement strains during recovery from 10 kGy acute IR or sham exposures. Knockdown of PprS does not impact D. radiodurans growth phenotype during unirradiated conditions. However, PprSKD demonstrates a significant reduction in growth rate during recovery from IR compared to WT. This growth defect after IR is lost after PprS complementation. *p-value < 0.05 for each comparison by two-tailed Student’s t-test.