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. 2021 Jun 21;11:12949. doi: 10.1038/s41598-021-91335-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PprSKD showed reduced survival to acute IR and reduced growth under both acute and chronic IR. (A) Survival curve of D. radiodurans PprS knockdown mutant (PprSKD), PprSKD with empty plasmid control (PprSKD_EV), PprS complementation (PprSCom) and WT at different doses of acute IR. Error bars represent standard deviation of biological triplicates and *p-value < 0.05 between the strain compared to WT by two-tailed Student’s t-test. (B) Growth of WT, PprS knockdown and complement strains, and a recA defect strain (rec30) under chronic irradiation at 57 Gy/h for 5 days. Numbers over the images indicate the dilution ratio of colonies. (C) Doubling time of biological triplicates of WT and PprS knockdown and complement strains during recovery from 10 kGy acute IR or sham exposures. Knockdown of PprS does not impact D. radiodurans growth phenotype during unirradiated conditions. However, PprSKD demonstrates a significant reduction in growth rate during recovery from IR compared to WT. This growth defect after IR is lost after PprS complementation. *p-value < 0.05 for each comparison by two-tailed Student’s t-test.