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. 2021 Jun 8;8:687113. doi: 10.3389/fmolb.2021.687113

FIGURE 3.

FIGURE 3

LncRNA MEF2C-AS1 was the target of miR-592. (A) The potential miRNAs that may bind to lncRNA MEF2C-AS1 was predicted by searching the DIANA tools and the differentially expressed miRNAs screened from TCGA database; (B) TCGA data showed the expression changes of miR-592 in CESC tissues; (C) Kaplan-Meier survival curve from TCGA described the overall survival; (D) qRT-PCR was performed to detect the miR-592 expression in cervical cancer cell lines (Caski, C-33A, SiHa, and HeLa) and normal cervical cell (Ect1/E6E7); (E) DIANA tool predicts the binding sites between lncRNA MEF2C-AS1 and miR-592; (F) luciferase activity of MEF2C-AS1-wt, MEF2C-AS1-mut upon the transfection of miR-592 mimics or mimics NC was evaluated by luciferase reporter assay; (G) The mRNA expression of miR-592 was detected in SiHa and HeLa cells by qRT-PCR after transfection with pc-MEF2C-AS1. *p < 0.05, compared with normal tissues (B); **p < 0.01, compared with Ect1/E6E7 group (D); **p < 0.01, compared with mimics NC group (F); **p < 0.01, compared with pc-NC group (G).