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. 2021 Jun 8;12:676627. doi: 10.3389/fimmu.2021.676627

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Copyright © 2021 Mazziotta, Lanzillotti, Torreggiani, Oton-Gonzalez, Iaquinta, Mazzoni, Gaboriaud, Touzé, Silvagni, Govoni, Martini, Tognon and Rotondo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dilutional linearity of the indirect ELISA. Optical Density (OD) response to serial dilutions (1:20, 1:80, 1:160, 1:320, 1:640, 1:1,280 and 1:2,560) of n=3 MCPyV-positive sera presenting known high ODs. Each dilution was assayed in triplicate for each MCPyV VP1 S and VP2 F peptide, and ODs and sera dilutions were compared by linear regression analysis. (A) Good correlation between ODs and dilutions was found for VP1 S peptide with an R² of 0.9781 (p<0.0001), 0.9925 (p<0.0001) and 0.9809 (p<0.0001) for samples #1, #2 and #3, respectively. (B) Good correlation between ODs and dilutions was found for VP2 F peptide, with an R² of 0.9793 (p<0.0001) for sample #1, 0.9851 (p<0.0001) for samples #2 and 0.9853 (p<0.0001) for samples #3.