Table 2.

Indirect ELISA intra-assay and inter-assay coefficient of variations (CVs).

Peptides	Groups
	Low	Medium	High
	(n=10)	(n=10)	(n=10)
S peptide
Intra-assay (n=3 replicates)
Mean	0.062	0.156	0.178
SD	0.006	0.051	0.057
CV (%)	5.03	7.28	7.45
Inter-assay (n=3 replicates)
Mean	0.063	0.161	0.186
SD	0.005	0.049	0.06
CV (%)	4.4	7.09	7.86
F peptide
Intra-assay (n=3 replicates)
Mean	0.077	0.259	0.57
SD	0.021	0.087	0.21
CV (%)	6.41	6.02	7.17
Inter-assay (n=3 replicates)
Mean	0.078	0.267	0.592
SD	0.022	0.092	0.216
CV (%)	5.65	8.05	8.85

Indirect ELISA intra-assay and inter-assay coefficient of variations (CVs) evaluated on three set of control sera with high (n=10), medium (n=10) and low (n=10) optical density levels, for both Merkel cell polyomavirus VP1 S and VP2 F peptides. SD, standard deviation; CV, coefficient of variation.