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. 2021 Jun 8;9:643060. doi: 10.3389/fcell.2021.643060

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Copyright © 2021 Schaberg, Theocharidis, May, Lessmann, Schroeder and Faissner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of RPTPβ/ζ/DSD-1-PG in the embryonic spinal cord. (A) Immunohistochemistry with the polyclonal antibody anti-phosphacan revealed the expression of the CSPGs RPTP-β/ζ and DSD-1-PG/phosphacan in E15.5 mouse spinal cord. No labeling could be observed outside the CNS. (A′) The monoclonal antibody 473HD recognizes a specific carbohydrate motif on CSPGs that is present on RPTP-β/ζ /DSD-1-PG. Immuno-positive structures could also be found outside the CNS, especially in the underlying cartilage. (A″) The overlay of both labels and the nuclear DAPI staining (in white) showed a co-localization in the spinal cord, but solely 473HD-positive structures outside the CNS. (B) Young neurons in the developing spinal cord could be labeled with βIII-tubulin which is present especially in the ventral marginal zone. There, the expression partially overlapped with 473HD staining (B′,B″) (C,C′,C″). The immune-positive signals for 473HD surrounded scattered Isl-1/2-positive nuclei in the ventral spinal cord but showed no reactivity in the dorsal root ganglia (DRG) where Isl-1/2-positive motoneuron nuclei were arranged. Scale bars: 200 μm.