Skip to main content
. 2021 Jun 8;12:676869. doi: 10.3389/fendo.2021.676869

Figure 2.

Figure 2

ISF promotes GLP-1 secretion and activates the nodose ganglion (NG) and the nucleus tractus solitarius (NTS) via capsaicin-sensitive sensory nerves. (A) Time course of total GLP-1 concentrations in portal vein plasma after po administration of 30 ml/kg ISF or control solution. n = 7. ** p < 0.01 by two-way ANOVA followed by Bonferroni’s test vs. control. # p < 0.05 by two-way ANOVA followed by Dunnett’s test vs. 0 min. (B–I) NG (B–E) and NTS (F–M) sections immunostained for pERK1/2 at 30 min after po administration of 30 ml/kg ISF or control solution in control and systemic capsaicin (CAP)-treated mice. (J–M) Extended pictures of red squares in (F–I), respectively. Arrowhead indicates pERK1/2-positive neurons. Scale bar, 100 µm. (N, O) Number of pERK1/2-positive neurons in the NG (N) and in the medial NTS (O). CAP(−); intact mice. CAP(+); CAP-treated mice. n = 5–6. * p < 0.05 and ** p < 0.01 by one-way ANOVA followed by Tukey’s test. Data show means ± SEM.