Figure 6.

Subchronic administration of ISF (20 ml/kg/day) on hyperphagic obesity in HFD-fed mice. (A–H) HFD-fed mice (HFD-fed), compared to lean mice fed a standard chow (Chow-fed), exhibited light period (LP)-selective hyperphagia accompanied by daily hyperphagia (A–F). Subchronic administration once daily at LP onset (7:30) of ISF (20 ml/kg/day), compared to control solution, for 10 days in HFD-fed obese mice significantly suppressed LP HFD intake (A, D) and daily HFD intake (C, F) but not dark period (DP) HFD intake (B, E), and lowed body weight gain (H). n = 5. Different letters indicate p < 0.05 by two-way ANOVA followed by Tukey’s test (A–H).