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. 2021 Jun 8;12:636954. doi: 10.3389/fimmu.2021.636954

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Copyright © 2021 Dömer, Walther, Möller, Behnen and Laskay

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NET-induced phosphorylation of p38, Akt and ERK1/2. Neutrophils were treated with PMA, NETs, fNETs or left untreated for 15 minutes. Phosphorylation of Akt, ERK1/2 and p38 were analyzed by Western Blot. (A–C) Representative Western blots. (D–F) Phosphorylation of Akt, ERK1/2 and p38 were quantified by densitometry analysis. Signals of phosphoproteins were related to the signal of corresponding unphosphorylated proteins. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3, *=p ≤ 0,05, **=p ≤ 0,01, ***=p ≤ 0,001, ****=p ≤ 0,0001. n.s, not significant; a.u., arbitrary units corresponding to the ratio of phosphorylated to non-phosphorylated proteins.