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. 2021 Jun 7;148(11):dev198044. doi: 10.1242/dev.198044

Fig. 4.

Fig. 4.

Synergistic effect of the neuron and muscle-specific expression of daf-16 in ‘ventral targeting’ and functional recovery. (A,B) RI values (A) and percentage of ‘ventral targeting’ events (B) in daf-16(lf) with or without the daf-16f transgene expressed under the endogenous promoter Pdaf-16 [muEx248], intestinal promoter Pges-1 [muIs142], pan neuronal promoter Prgef-1 [shrEx318], touch neuron-promoter Pmec-4 [shrEx226], muscle-promoter Pmyo-3 [shrEx314] and epidermal promoter Pdpy-7 [shrEx316]. The transgene shrEx320 was used to co-express daf-16f in touch neuron and muscle. A two-segmented y-axis was used in plot A. For A and B, N (independent replicates)=3-9. (C) The depth-coded images of ‘ventral targeting’ events at 24 h post-axotomy performed in daf-16(lf) and daf-16(lf);Pmec-4::daf-16f [shrEx226] background at L4 stage. The double-headed arrow represents regrowth along the VNC. (D) The longitudinal regrowth along the VNC at 24 h post-axotomy performed with L4 worms in wild type (WT) and daf-16(lf) with or without rescue transgenes. shrEx226 and shrEx314 was used to drive daf-16f in touch neuron and muscle, respectively. N=3-5. (E) Confocal images and illustrations of the regrowth patterns seen due to the expression of Pmec-4::dlk-1[+] in the wild-type [shrEx389] and daf-16(lf) [shrEx391] backgrounds at 24 h post-axotomy. The arrowheads represent regrowth from the proximal stump. The red arrow indicates the site of injury. (F,H) Regrowth length (F), percentage of ‘ventral targeting’ (G) and RI values (H) at 24 h post-axotomy in wild-type and daf-16(lf) backgrounds, as shown in E. N=2-4. Data are mean±s.d. *P<0.05; **P<0.01; ***P<0.001; ns, non-significant [ANOVA with Tukey's multiple comparisons test (A,D,F,H); Fisher's exact test (B,G)]. Data are mean±s.d.