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. 2021 Jun 7;148(11):dev198044. doi: 10.1242/dev.198044

Fig. 6.

Fig. 6.

IIS receptor and DAF-16 regulate unc-6 expression in the ventral muscle. (A) Confocal image with or without the differential interference contrast (DIC) channel of worms at L4 and A3 stages showing the expression of unc-6(fosmid)::SL2::NLS::YFP::H2B in the nuclei of ventral muscles (arrowheads) shown as M1 to M8. (B-D) Confocal images showing unc-6 expression in wild type (WT) (B), daf-2(e1368ts) (C) and daf-2(e1368ts); daf-16(lf) (D) in day 3 (A3) adults. (E) The expression of unc-6 in the background of the Pmyo-3::daf-16f [shrEx313] transgene at L4 stage. The insets show the enlarged images of the nuclei of M1, M2 and M8 muscles. The ventral muscles are shown using the dark and light red color outlines. (F) The number of visible nuclei showing the localization of unc-6::YFP at L4 and A3 stages. N (independent replicates)=3-5. (G) The mean intensity of unc-6::YFP reporter in nuclei of M1, M2 and M8 muscles at L4 and A3 stages. N=3-5. (H) The mean intensity of unc-6 reporter in the nuclei of M1, M2 and M8 muscles in wild-type, daf-2(e1368ts) and daf-2(e1368ts); daf-16(mu86) worms imaged at the A3 stage. N=4-7. (I) The mean intensity of UNC-6::YFP in the nuclei of M1 and M2 muscles in wild-type, daf-16(lf) and Pmyo-3::daf-16f (shrEx313) backgrounds. N=3-6. Data are mean±s.d. *P<0.05; **P<0.01; ***P<0.001; ns, non-significant [Mann–Whitney unpaired non-parametric two-tailed Student's t-test (F,G); ANOVA with Tukey's multiple comparisons test (H,I)]. a.u., arbitrary units.