Table II.
Parameters of Calibration Curves for Individual Lipid Classes and Lipid Species Added into the Egg Lipid Extract; Experimentally Determined Response Factors (RF) Used for Relative Quantification of Lipid Classes
| Compound | Lipid class | Linear range ug/mL | Slope, a | Intercept, b | R2 | RF |
|---|---|---|---|---|---|---|
| 1Cholesterol | FC | 0.05-10 | 0.58 | 0.02 | 0.994 | 72.64 |
| 1PE 18:0/18:0 | PE | 0.2–40 | 0.17 | 0.18 | 0.992 | 3.59 |
| 1LPE 17:1/0:0 | LPE | 2.5–10 | 0.61 | -0.50 | 0.997 | 23.69 |
| 1PC 18:0/18:0 | PC | 0.2–10 | 0.66 | -0.07 | 0.998 | 1.00 |
| PC P-18:0/18:1 | PC(P) | 0.2–10 | 0.57 | -0.06 | 0.997 | 1.16 |
| 1SM d16:0/18:1 | SM | 0.2–10 | 0.39 | -0.12 | 0.998 | 1.98 |
| 1LPC 16:0/0:0 | LPC | 0.8–10 | 0.30 | -0.27 | 0.993 | 4.40 |
| 1PG 16:0/18:1 | PG | 0.8–38.7 | 0.31 | 0.33 | 0.992 | 41.77 |
| 1TG 18:0/18:0/18:0 | TG | 0.05–2.5 | 1.19 | -0.03 | 0.992 | 0.29 |
| TG 18:2/18:2/18:2 | TG | 0.1–5 | 0.87 | 0.06 | 0.994 | 0.30 |
| 1DG 18:1/18:1/0:0 | DG | 0.05–2.5 | 1.02 | 0.04 | 0.995 | 0.35 |
| DG 18:1/0:0/18:1 | DG | 0.05–2.5 | 1.10 | 0.16 | 0.983 | 0.39 |
| 1MG 18:1/0:0/0:0 | MG | 0.05–2.5 | 0.93 | 0.06 | 0.997 | 1.25 |
| 1Cer d18:1/17:0 | Cer | 0.05–1.25 | 1.38 | 0.09 | 0.982 | 0.32 |
| GlcCer d18:1/17:0 | HexCer | 0.05–2.5 | 0.53 | -0.02 | 0.999 | 0.68 |
| 1GalCer d18:1/18:0 | HexCer | 0.05–10 | 0.56 | 0.05 | 0.998 | 0.56 |
| 1CE 18:1 | CE | 0.2–5 | 0.60 | 0.20 | 0.989 | 1.70 |
1Calibration curves used for quantification of lipid classes.