Table III.
Egg Lipid Extract Spiked with a Known Amount of Standards; Comparison of Single-Point Internal Standard Method and Multiple-Point Internal Standard Method
| Lipid compound | tR (min) | Amount added (ug/ml) | Single-Point | Multiple-Point | Accuracy (dev %) | |||
|---|---|---|---|---|---|---|---|---|
| Mean | RSD | Mean | RSD | Single point | Multiple point | |||
| CE 18:1 | 0.61 | 0.53 | 1.51 | 0.8 | 0.29 | 1.8 | 183.1 | 44.8 |
| 2.13 | 5.36 | 3.6 | 1.90 | 4.2 | 151.1 | 10.8 | ||
| 4.27 | 9.27 | 1.2 | 3.54 | 1.3 | 117.4 | 17.1 | ||
| TG 18:0/18:0/18:0 | 0.93 | 0.13 | 0.12 | 1.6 | 0.13 | 1.3 | 8.5 | 4.9 |
| 0.53 | 0.47 | 1.5 | 0.42 | 1.5 | 12.0 | 21.7 | ||
| 1.07 | 0.93 | 12.9 | 0.80 | 12.5 | 13.3 | 25.1 | ||
| TG 18:1/18:1/18:1 | 0.95 | 0.13 | 0.11 | 2.1 | 0.06 | 4.6 | 16.5 | 56.1 |
| 0.53 | 0.46 | 2.1 | 0.46 | 2.4 | 14.2 | 14.4 | ||
| 1.07 | 0.91 | 14.1 | 0.98 | 15.1 | 14.6 | 13.4 | ||
| TG 18:2/18:2/18:2 | 0.99 | 0.10 | 0.09 | 2.3 | 0.04 | 6.7 | 7.8 | 63.5 |
| 0.40 | 0.36 | 1.4 | 0.34 | 1.7 | 10.5 | 14.6 | ||
| 0.80 | 0.72 | 11.0 | 0.76 | 12.0 | 11.0 | 10.5 | ||
| DG 18:1/18:1/0:0 | 1.36 | 0.15 | 0.12 | 2.1 | 0.08 | 3.0 | 15.2 | 42.2 |
| 0.59 | 0.52 | 1.3 | 0.47 | 1.4 | 11.8 | 20.2 | ||
| 1.17 | 0.96 | 1.0 | 0.90 | 1.0 | 17.9 | 22.9 | ||
| DG 18:1/0:0/18:1 | 1.25 | 0.10 | 0.14 | 13.7 | NA | NA | 43.7 | NA |
| 0.40 | 0.57 | 7.6 | 0.37 | 10.7 | 42.0 | 8.3 | ||
| 0.80 | 1.08 | 3.0 | 0.84 | 3.6 | 35.6 | 4.6 | ||
| MG 18:1/0:0/0:0 | 1.63 | 0.19 | 0.19 | 2.6 | 0.13 | 4.0 | 1.9 | 29.6 |
| 0.75 | 0.70 | 8.8 | 0.68 | 9.7 | 6.8 | 9.2 | ||
| 1.49 | 1.18 | 3.2 | 1.20 | 3.4 | 20.9 | 19.8 | ||
| Cer d18:1/17:0 | 2.03 | 0.10 | 0.13 | 8.8 | 0.03 | 28.6 | 31.8 | 70.5 |
| 0.40 | 0.54 | 0.4 | 0.33 | 0.4 | 35.8 | 17.9 | ||
| 0.80 | 0.91 | 3.0 | 0.59 | 3.4 | 13.1 | 26.1 | ||
| GlcCer d18:1/17:0 | 3.03 | 0.13 | 0.06 | 3.1 | 0.15 | 2.4 | 53.8 | 10.1 |
| 0.53 | 0.23 | 0.1 | 0.46 | 0.1 | 57.7 | 14.4 | ||
| 1.07 | 0.43 | 3.1 | 0.85 | 2.9 | 59.6 | 20.8 | ||
| GalCer d18:1/18:0 | 3.11 | 0.12 | 0.05 | 3.5 | 0.01 | 37.6 | 56.5 | 92.7 |
| 0.48 | 0.22 | 0.3 | 0.31 | 0.4 | 54.6 | 36.0 | ||
| 0.96 | 0.43 | 2.2 | 0.69 | 2.5 | 55.0 | 27.8 | ||
| PG 16:0/18:1 | 3.60 | 0.72 | 0.72 | 3.1 | NA | NA | 2.2 | NA |
| 2.89 | 4.04 | 3.6 | 2.20 | 5.3 | 39.7 | 23.9 | ||
| 5.78 | 7.26 | 1.0 | 4.82 | 1.2 | 25.7 | 16.5 | ||
| PE 18:0/18:0 | 3.89 | 0.51 | 0.37 | 0.7 | 0.50 | 0.7 | 27.2 | 1.0 |
| 2.03 | 1.64 | 0.9 | 2.23 | 0.9 | 19.2 | 10.0 | ||
| 4.05 | 3.12 | 0.4 | 4.24 | 0.4 | 23.1 | 4.6 | ||
| LPE 17:1/0:0 | 4.40 | 0.48 | 0.96 | 11.7 | 1.21 | 3.8 | 99.0 | 152.6 |
| 1.92 | 4.08 | 1.7 | 2.49 | 1.1 | 112.3 | 29.5 | ||
| 3.84 | 7.25 | 1.4 | 3.78 | 1.1 | 88.8 | 1.5 | ||
| PC 18:0/18:0 | 4.76 | 0.40 | 0.37 | 1.8 | 0.33 | 1.3 | 8.2 | 16.3 |
| 1.60 | 1.56 | 0.2 | 1.08 | 0.1 | 2.3 | 32.2 | ||
| 3.20 | 2.99 | 0.7 | 1.98 | 0.7 | 6.5 | 38.1 | ||
| PC P-18:0/18:1 | 4.78 | 0.19 | 0.18 | 1.5 | 0.23 | 0.8 | 4.9 | 21.8 |
| 0.75 | 0.72 | 0.4 | 0.63 | 0.4 | 3.0 | 16.0 | ||
| 1.49 | 1.38 | 0.4 | 1.11 | 0.4 | 7.3 | 25.8 | ||
| PC 18:3/18:3 | 4.82 | 0.59 | 0.91 | 0.4 | 0.68 | 0.3 | 55.7 | 15.5 |
| 2.35 | 4.07 | 0.9 | 2.65 | 0.9 | 73.2 | 13.1 | ||
| 4.69 | 7.68 | 0.6 | 4.92 | 0.6 | 63.7 | 4.9 | ||
| LPC 16:0/0:0 | 5.06 | 0.43 | 0.20 | 0.3 | 1.10 | 0.0 | 52.1 | 157.0 |
| 1.71 | 1.04 | 0.7 | 1.80 | 0.4 | 39.0 | 5.7 | ||
| 3.41 | 2.11 | 1.4 | 2.71 | 0.9 | 38.1 | 20.7 | ||
| Cholesterol | 1.36 | 1.07 | 1.17 | 2.7 | 0.23 | 3.0 | 9.8 | 78.8 |
| 4.27 | 4.40 | 0.8 | 0.92 | 0.8 | 3.1 | 78.3 | ||
| 8.53 | 8.40 | 2.0 | 1.79 | 2.1 | 2.1 | 79.0 | ||
n = 3.