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. 2021 Jun 8;9:663959. doi: 10.3389/fcell.2021.663959

FIGURE 4.

FIGURE 4

Downregulation of Lnc-FR332443 and Runx1 expression during adipogenic differentiation. (A) Oil red O staining of mouse 3T3-L1 preadipocyte adipogenic differentiation at days 0, 2, 4, 6, and 8. Scale bar, 200 μm. (B) The Lnc-FR332443 and Runx1 mRNA levels during 3T3-L1 preadipocyte differentiation on different days. (C) Western blot analysis of Runx1 and adipogenic marker (anti-PPARγ and anti-C/EBPα) protein levels in 3T3-L1 preadipocytes differentiated on different days. (D) The quantification data of panel (C). (E) The time course of Lnc-FR332443 and Runx1 mRNA expression during 3T3-L1 preadipocyte differentiation after infection with lentivirus-Lnc-FR332443. (F) Western blots of Runx1 protein expression in the 3T3-L1 preadipocytes differentiated on different days after lentivirus-Lnc-FR332443 infection. (G) The quantification data of panel (F). All the data are shown as the mean ± SEM, NS denotes not significant, N = 3, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.