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. 2021 Jun 8;9:663959. doi: 10.3389/fcell.2021.663959

FIGURE 5.

FIGURE 5

Lnc-FR332443 inhibits 3T3-L1 preadipocyte differentiation in vitro. (A) Oil red O staining of 3T3-L1 preadipocytes transfected with Lnc-FR332443 siRNA and its control at day 4. Scale bar, 200 μm. (B) The protein expression levels of Runx1 and the adipogenic markers PPARγ, C/EBPα and FABP4 in the 3T3-L1 cells transfected with Lnc-FR332443 siRNA and its control. (C) Quantification data of panel (B). (D) The mRNA expression levels of Lnc-FR332443, Runx1, PPARγ, C/EBPα and FABP4 in the 3T3-L1 cells transfected with Lnc-FR332443 siRNA and its control. All data are presented as the mean ± SEM, NS denotes not significant, N = 4, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.001.