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. 2021 Jun 8;12:625922. doi: 10.3389/fimmu.2021.625922

Figure 1.

Figure 1

Isolation of a high purity population of low density neutrophils from healthy donors (A) Overview of traditional isolation protocol PBMC, peripheral blood mononuclear cells PMN, polymorphonuclear cells; RBC, red blood cells. (B) Representative images of cells isolated by traditional protocol. Black arrows show neutrophils in PBMC layer. (C) Overview of magnetic, negative isolation protocol, LDN, low density neutrophils; NDN, normal density neutrophils. (D) Representative images of enriched LDNs and NDNs isolated by magnetic, negative selection protocol (E) Quantification of neutrophils isolated by each protocol, (N=5) (F) Total number of neutrophils (CD66b+ CD16+) identified within the PBMC population isolated from 12 mls blood by flow cytometry (N=5) (G) Total number of neutrophils isolated from 12mls blood by magnetic isolation (N=10). Statistical testing with two tailed unpaired T test where **p ≤ 0.01 Data shows means ± SEM error bars.