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. 2021 Jun 8;12:625922. doi: 10.3389/fimmu.2021.625922

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Copyright © 2021 Hardisty, Llanwarne, Minns, Gillan, Davidson, Gwyer Findlay and Gray

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NDNs become LDNs upon activation with inflammatory stimuli. (A) Diagram of method. PMN isolated by traditional methods are activated in vitro with inflammatory stimuli and then undergo repeat Percoll gradient. (B) Number of neutrophils recovered from the ‘PMN/NDN’ layer after second Percoll gradient. (N=4) (C) Number of neutrophils recovered from the ‘PBMC/LDN’ layer after second Percoll gradient. (N=4) (D) Representative cytospin images of neutrophils recovered in each condition. Statistical analysis by One-way ANOVA with multiple comparisons where ***p ≤ 0.001.