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LDNs are not elevated in people with Cystic Fibrosis. (A) Total numbers of LDNs isolated from 12 mls blood by magnetic separation and Percoll gradient. (N=5) (B) Rate of apoptosis after in vitro culture for 20 hrs, measured by Annexin V and Propidium Iodide staining. (N=4-6) (C) NET formation, measured by SYTOX green fluorescence over 6 hours after stimulation with 100nM PMA (N=5-6) Data shown as mean ± SEM error bars.
