Table 3.

Overview of LDN population isolation and identification.

Disease/Condition:		LDN isolation/identification method:	Authors, Year:
Autoimmunity	Systemic Lupus Erythematosus	Ficoll Density	Hacbarth & Kadjdacsy-Balla (2)
		Count
	Systemic Lupus Erythematosus	CPT- Heparin tube	Rahman et al. (7)
		RBC lysis
		Negative magnetic bead selection
	Systemic Lupus Erythematosus	Heparin tube	Denny et al. (25)
		RBC lysis
		Negative magnetic bead selection
	Systemic Lupus Erythematosus	Heparin tube	Villanueva et al. (23)
		RBC lysis
		Negative magnetic bead selection
	Rheumatoid Arthitis	Hetasep solution	Wright et al. (30)
		Ficoll Density
		Negative magnetic bead isolation
	ANCA Vasculitis	Dextran sedimentation	Ui Mhaonaigh et al. (40)
		Percoll Density
		RBC lysis
		Flow cytometry
Cancer	Advanced Lung Adenocarcinoma	Ficoll Density	Liu et al. (3)
		Flow cytometry
	Cancer	Dextran sedimentation	Sagiv et al. (8)
		Histopaque 1077
		Flow cytometry
	Late stage Lung Cancer	Dextran sedimentation	Shaul et al. (29)
		Histopaque 1077
		Flow cytometry
		Negative magnetic bead isolation
Infection	Human Immunodeficiency Virus	Histopaque 1077	Cloke et al. (13)
		Dextran sedimentation
		CD15+ isolation
	Bunyavirus	Ficoll Density	Li et al. (10)
		RBC lysis
		Flow cytometry
	Severe bacterial infection	Ficoll Density	Morisaki et al. (11)
		RBC lysis
		Percoll Density
	Mycobacterium Tuberculosis	LDN:
		Lymphocyte Separation Medium	Deng et al. (12)
		CD14- CD15+ FACS
		NDN:
		Dextran sedimentation
		Ficoll Density
	Mycobacterium Tuberculosis	Ficoll Density	Su et al. (24)
		RBC lysis
		Negative magnetic bead isolation
		Flow cytometry
	Mycobacterium Tuberculosis	LDN:	La Manna et al. (26)
		Lymphocyte Separation Medium
		CD14- CD15+ FACS
		NDN:
		Ficoll Density
		RBC lysis
	Malaira	LDN:	Rocha et al. (18)
		Ficoll Density
		CD66b+ positive selection
		NDN:
		Ficoll Density
		Negative magnetic bead isolation
		Flow cytometry
Pregnancy	Pregnancy/cord blood	Histopaque 1077	Ssemaganda et al. (5)
		RBC lysis
		Flow cytometry
Surgery	Laparotimy (Lavage)	Ficoll Density	Kanamaru et al. (9)
		LDN: CD66b+ positive isolation
	Abdominal surgery (Blood)	Dextran sedimentation	Kumagi et al. (27)
		Ficoll Density
		LDN: CD66b+ positive isolation
Inflammation	Psoriasis	Ficoll Density	Teague et al. (14)
		RBC lysis
		Flow cytometry
	Idiopathic Inflammatory Myopathy	Ficoll Density	Seto et al. (15)
		RBC lysis
		Flow cytometry
	Adult Onset Still’s Disease	Lymphoprep	Torres-Ruiz et al. (20)
		Flow cytometry
	Juvenille Ioiopathic Arthritis	Hetasep solution	Ronchezel et al. (41)
		Histopaque 1077
		RBC lysis
		Flow cytometry
Allergy	Asthma	Lymphocyte Separation Medium	Fu et al. (33)
		LDN: Flow cytometry
		NDN: RBC lysis