Figure 9.

Validation of biological processes: validation was done by using Licor 6400XT (LI-COR, Lincoln, NE, USA) on 21-day-old grown plants. Plants were grown in garden soil and fertilized with Arnon–Hoagland medium having nitrate as the sole source of N with 15 mM N concentration as control while 1.5 mM was used as a test. Measurement was done in five biological (A) Photosynthesis was measured in terms of μ mol CO₂/m²s¹, (B) Transpiration was measured in terms of mol (H₂O)/m²s¹, (C) Stomatal conductance was measured in terms of mol(H₂O) m⁻² sec⁻¹, (D) Internal water-use efficiency was measured in terms of μ mol CO₂/mol(H₂O), and (E) Transpiration efficiency was measured in terms of μ mol CO₂/m mol H₂O/m²/s. The different alphabets over bars show their statistical significance.