. 2021 May 26;11(12):6763–6770. doi: 10.1021/acscatal.1c00996

Table 3. Michaelis–Menten Kinetic Parameters Calculated for a Single Substrate, 1a, for the Friedel–Crafts Reaction between 1a and 2a to Give 3a^a.

mutant	K_M-1a, app (mM)	k_cat, app (s^–1) × 10²	(k_cat/K_M)_app (M^–1s^–1)	vs LmrR_pAF
LmrR_pAF	18.2 ± 2.8	1.45 ± 0.11	0.80 ± 0.06	1
LmrR_pAF_RGN	5.2 ± 0.9	0.64 ± 0.03	1.25 ± 0.17	1.56
LmrR_pAF_RMH	25.7 ± 6.6	0.27 ± 0.04	0.11 ± 0.02	0.14

Determined at 25 °C in phosphate buffer (50 mM) containing NaCl (150 mM) and 5% (v/v) DMF at pH 6.5. The estimated errors reflect the standard deviation from three independent experiments. 2a was kept at a concentration of 1 mM, and 1a was varied between 2 mM and 30 mM.

Table 3. Michaelis–Menten Kinetic Parameters Calculated for a Single Substrate, 1a, for the Friedel–Crafts Reaction between 1a and 2a to Give 3aa.

Table 3. Michaelis–Menten Kinetic Parameters Calculated for a Single Substrate, 1a, for the Friedel–Crafts Reaction between 1a and 2a to Give 3a^a.