. 2021 May 26;11(12):6763–6770. doi: 10.1021/acscatal.1c00996

Table 4. Michaelis–Menten Kinetic Parameters Calculated for a Single Substrate, 5, for the Hydrazone Formation Reaction between 4 and 5 to Give 6^a.

mutant	K_M-5, app (μM)	k_cat, app (s^–1) × 10³	(k_cat/K_M)_app (M^–1s^–1)	vs LmrR_pAF
LmrR_pAF^b	122 ± 10	0.23 ± 0.01	1.85 ± 0.08	1
LmrR_pAF_RGN	203 ± 87	0.11 ± 0.03	0.56 ± 0.17	0.30
LmrR_pAF_RMH^b	38 ± 4	3.98 ± 0.16	105 ± 7	56.7

Determined at 25 °C in phosphate buffer (50 mM) containing NaCl (150 mM) and 5% (v/v) DMF at pH 7.4. The estimated errors reflect the standard deviation from three independent experiments. 4 was kept at a constant concentration of 5 mM, and the concentration of 5 was varied from 20 to 200 μM.

Data from ref (42).

Table 4. Michaelis–Menten Kinetic Parameters Calculated for a Single Substrate, 5, for the Hydrazone Formation Reaction between 4 and 5 to Give 6a.

Table 4. Michaelis–Menten Kinetic Parameters Calculated for a Single Substrate, 5, for the Hydrazone Formation Reaction between 4 and 5 to Give 6^a.